Several studies have shown that bactericidal antibiotics induce cell death through a common oxidative damage mechanism based on reactive oxygen species (ROS) production. The mechanism relies on activated cellular respiration in the presence of antibiotics. Working with a fluorogenic α-tocopherol analogue, a lipid peroxyl radical sensor probe, we recently showed that bacterial membrane damage in the presence of antibiotics - via ROS-induced lipid peroxidation - is implicated in bacterial cell death. Notably, this BODIPY-based sensor, once activated upon trapping membrane peroxyl radicals, was observed to under switching between on and off states. This switching proved instrumental toward allowing super resolution imaging of bacterial membrane sites where antibiotic induced peroxidation took place. In this presentation, I will describe mechanistic studies as well as benchmarking of the performance of this new probe toward doing super-resolution fluorescence imaging of lipid membranes. Concepts such as photostability, photon budget, photons per pulse, on and off dwell times, etc. will be described. I will next provide results on ciprofloxacin induced membrane peroxidation in gram negative bacteria E. coli and P. aeruginosa. These super-resolution imaging studies on the bactericidal antibiotic induced membrane peroxidation yield fundamental knowledge on ROS production and associated lethality in bacteria. We posit that this understanding may prove critical in developing new therapies to combat the emergence of antibiotic resistant strains.